Final Wash Buffer (Item J): 10ml vial of 10x concentrated buffer
Prepare all reagents, samples and standards as instructed
Add 25µl standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4°C
Add 25 µl detection affinity reagent to each well. Incubate 1 hour at room temperature
Add 15µL Primer solution to each well
Add 10µL of PCR Master Mix to each well
Run real-time PCR
May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2° to 8°C. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.