RayBio® Human Visfatin ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
Uniprot Accession Number: P43490 Gene Symbol: NAMPT I PBEF I PBEF1 Gene ID: 10135 Protein Name/synonyms: Nicotinamide phosphoribosyltransferase (NAmPRTase) (Nampt) (EC 18.104.22.168) (Pre-B-cell colony-enhancing factor 1) (Pre-B cell-enhancing factor) (Visfatin)
Target species: Human Solid support: 96-well Microplate Solid support: Quantitative Result Output: Quantitative Method of Detection: Colorimetric Specificity: This ELISA antibody pair recognizes .
Strip plates and additional reagents allow for use in multiple experiments
Quantitative protein detection
Establishes normal range
The best products for confirmation of antibody array data
Pre-Coated 96-well Strip Microplate
Biotinylated Detection Antibody
TMB One-Step Substrate
Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4°C.
Add 100 µl of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 µl of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.