RayBio® Human Trypsin Pan/PRSS1-2-3 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
Uniprot Accession Number: P07477 P07478 P35030 Gene Symbol: PRSS1 I TRP1 I TRY1 I TRYP1 PRSS2 I TRY2 I TRYP2 PRSS3 I PRSS4 I TRY3 I TRY4 Gene ID: 5644 5645 5646 Protein Name/synonyms: Trypsin-1 (EC 22.214.171.124) (Beta-trypsin) (Cationic trypsinogen) (Serine protease 1) (Trypsin I) [Cleaved into: Alpha-trypsin chain 1 Alpha-trypsin chain 2] Trypsin-2 (EC 126.96.36.199) (Anionic trypsinogen) (Serine protease 2) (Trypsin II) Trypsin-3 (EC 188.8.131.52) (Brain trypsinogen) (Mesotrypsinogen) (Serine protease 3) (Serine protease 4) (Trypsin III) (Trypsin IV)
Target species: Human Solid support: 96-well Microplate Solid support: Quantitative Result Output: Quantitative Method of Detection: Colorimetric Compatible Sample Types: Cell Culture Supernatants/Plasma/Serum Sensitivity: 30 pg/ml Detection Range: 30.72 pg/ml - 7500 pg/ml Recommended Dilution (serum & plasma): 200 fold Specificity: This ELISA antibody pair recognizes Human Trypsin Pan.
Spiking and Recovery Data
Strip plates and additional reagents allow for use in multiple experiments
Quantitative protein detection
Establishes normal range
The best products for confirmation of antibody array data
Pre-Coated 96-well Strip Microplate
Biotinylated Detection Antibody
TMB One-Step Substrate
Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4°C.
Add 100 µl of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 µl of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.