Human WISP-1 (CCN4) ELISA Kit for cell culture supernatants, EDTA treated plasma, and serum samples. Heparin and citrate are not recommended.
Uniprot Accession Number: O95388 Gene Symbol: WISP1 I CCN4 Gene ID: 8840 Protein Name/synonyms: WNT1-inducible-signaling pathway protein 1 (WISP-1) (CCN family member 4) (Wnt-1-induced secreted protein)
Target species: Human Solid support: 96-well Microplate Solid support: Quantitative Result Output: Quantitative Method of Detection: Colorimetric Compatible Sample Types: Cell Culture Supernatants I Plasma I Serum Sensitivity: 50 pg/ml Detection Range: 50 pg/ml - 15000 pg/ml Recommended Dilution (serum & plasma): 2 fold Specificity: In this sandwich ELISA format, no cross-reactivity was observed with rhBiglycan, rhDecorin, rhNOV, or rhWISP-3.
Serial Dilution Series
Spiking and Recovery Data
Strip plates and additional reagents allow for use in multiple experiments
Quantitative protein detection
Establishes normal range
The best products for confirmation of antibody array data
Pre-Coated 96-well Strip Microplate
Biotinylated Detection Antibody
TMB One-Step Substrate
Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4°C.
Add 100 µl of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 µl of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.