RayBio® Human ANGPTL3 ELISA Kit for serum, plasma, and cell culture supernatant samples.
Uniprot Accession Number: Q9Y5C1 Gene Symbol: ANGPTL3 I ANGPT5 I UNQ153/PRO179 Gene ID: 27329 Protein Name/synonyms: Angiopoietin-related protein 3 (Angiopoietin-5) (ANG-5) (Angiopoietin-like protein 3)
Target species: Human Solid support: 96-well Microplate Solid support: Quantitative Result Output: Quantitative Method of Detection: Colorimetric Compatible Sample Types: Cell Culture Supernatants I Plasma I Serum Sensitivity: 8.2 pg/ml Detection Range: 8.2 pg/ml - 2000 pg/ml Recommended Dilution (serum & plasma): 200 fold Specificity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human beta IG-H3, CA9, Cathepsin B, CD23, CD26 (DPPIV), CHI3L1, Coagulation Factor III, CTLA-4, Dkk-4, EDA-A2, Erythropoietin R, FGF-6, FGF-9, FOLH1 (PSMA), Gas 1, IGFBP-5, IL-38 (IL-1 F10), IL-36 Ra (IL-1 F5), IL-36 alpha (IL-1 F6), IL-37 (IL-1 F7), IL-36 beta (IL-1 F8), IL-36 gamma (IL-1 F9), IL-1 R5 (IL-18 R alpha), IL-10 R alpha, IL-17C, IL-18, IL-20, IL-34, IL-5 R alpha, Layilin, Leptin R, Marapsin, Mer, MMP-7, P-Cadherin, Prostasin, SIGIRR, TGF beta R3,TWEAK.
Spiking and Recovery Data
Jurkat cells were treated with Calyculin A & PV. Cell lysates were analyzed using this phosphoELISA and Western Blot.
Strip plates and additional reagents allow for use in multiple experiments
Quantitative protein detection
Establishes normal range
The best products for confirmation of antibody array data
Pre-Coated 96-well Strip Microplate
Biotinylated Detection Antibody
TMB One-Step Substrate
Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4°C.
Add 100 µl of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 µl of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.