RayBio® Human Amnionless ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
Uniprot Accession Number: Q9BXJ7 Gene Symbol: AMN I UNQ513/PRO1028 Gene ID: 81693 Protein Name/synonyms: Protein amnionless
Target species: Human Solid support: 96-well Microplate Solid support: Quantitative Result Output: Quantitative Method of Detection: Colorimetric Compatible Sample Types: Cell Culture Supernatants///Plasma///Serum Sensitivity: 21 pg/ml Detection Range: 21 pg/ml - 5000 pg/ml Recommended Dilution (serum & plasma): 2 fold Specificity: This ELISA antibody pair recognizes Human Amnionless.
Spiking and Recovery Data
HeLa cells were treated or untreated with Calyculin A. Cell lysates were analyzed using this phosphoELISA and Western Blot.
Strip plates and additional reagents allow for use in multiple experiments
Quantitative protein detection
Establishes normal range
The best products for confirmation of antibody array data
Pre-Coated 96-well Strip Microplate
Biotinylated Detection Antibody
TMB One-Step Substrate
Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4°C.
Add 100 µl of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 µl of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.