RayBio® Human Alpha-2 Macroglobulin ELISA Kit for cell culture supernatants, plasma, and serum samples.
Uniprot Accession Number: P01023 Gene Symbol: A2M I CPAMD5 I FWP007 Gene ID: 2 Protein Name/synonyms: Alpha-2-macroglobulin (Alpha-2-M) (C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5)
Target species: Human Solid support: 96-well Microplate Solid support: Quantitative Result Output: Quantitative Method of Detection: Colorimetric Compatible Sample Types: Cell Culture Supernatants I Plasma I Serum Sensitivity: 4 ng/ml Detection Range: 4 ng/ml - 3,000 ng/ml Recommended Dilution (serum & plasma): 2,000 fold Specificity: This ELISA antibody pair detects human Alpha 2-M. Other species not determined.
Serial Dilution Series
Spiking and Recovery Data
HeLa cells were untreated or treated with 0.1 µg/ml Calyculin A for 30 min. Cell lysates were analyzed using this phosphoELISA and Western Blot.
Strip plates and additional reagents allow for use in multiple experiments
Quantitative protein detection
Establishes normal range
The best products for confirmation of antibody array data
Pre-Coated 96-well Strip Microplate
Biotinylated Detection Antibody
TMB One-Step Substrate
Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4°C.
Add 100 µl of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 µl of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.