RayBio® Human 2B4 (CD244) ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
Uniprot Accession Number: Q9BZW8 Gene Symbol: CD244 I 2B4 Gene ID: 51744 Protein Name/synonyms: Natural killer cell receptor 2B4 (NK cell activation-inducing ligand) (NAIL) (NK cell type I receptor protein 2B4) (NKR2B4) (h2B4) (SLAM family member 4) (SLAMF4) (Signaling lymphocytic activation molecule 4) (CD antigen CD244)
Target species: Human Solid support: 96-well Microplate Solid support: Quantitative Result Output: Quantitative Method of Detection: Colorimetric Compatible Sample Types: Cell Culture Supernatants I Plasma I Serum Sensitivity: 0.05 ng/ml Detection Range: 0.05 ng/ml - 12 ng/ml Recommended Dilution (serum & plasma): 2 fold Specificity: The antibody pair provided in this kit recognizes human 2B4/CD244/SLAMF4.
Serial Dilution Series
Spiking and Recovery Data
HeLa cells were untreated or treated with UV. Cell lysates were analyzed using this phosphoELISA and Western Blot.
Strip plates and additional reagents allow for use in multiple experiments
Quantitative protein detection
Establishes normal range
The best products for confirmation of antibody array data
Pre-Coated 96-well Strip Microplate
Biotinylated Detection Antibody
TMB One-Step Substrate
Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4°C.
Add 100 µl of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 µl of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.