RayBio® Human/Mouse/Rat Visfatin EIA Kit optimized for serum and cell culture medium. Competition-based ELISA on a 96-well strip plate.
Uniprot Accession Number: P43490 Gene Symbol: NAMPT I PBEF I PBEF1 Gene ID: 10135 Protein Name/synonyms: Nicotinamide phosphoribosyltransferase (NAmPRTase) (Nampt) (EC 18.104.22.168) (Pre-B-cell colony-enhancing factor 1) (Pre-B cell-enhancing factor) (Visfatin)
Target species: Human I Mouse I Rat Solid support: 96-well Microplate Solid support: Quantitative Result Output: Quantitative Method of Detection: Colorimetric Compatible Sample Types: Cell Culture Supernatants I Serum Sensitivity: 0.778 ng/ml or 6.82 pM Detection Range: 0.1-1,000 ng/ml Recommended Dilution (serum & plasma): Human: 4X / Mouse: 4X / Rat: 2X Specificity: Cross Reactivity: This EIA kit shows no cross-reactivity with any of the cytokines tested: Ghrelin, Nesfatin, NPY and APC.
Strip plates and additional reagents allow for use in multiple experiments
Quantitative protein detection
Establishes normal range
The best products for confirmation of antibody array data
Pre-Coated 96-well Strip Microplate
TMB One-Step Substrate
Positive Control Sample
Prepare all reagents, samples and standards as instructed.
Add 100 µl detection antibody to each well.
Incubate 1.5 h at RT or O/N at 4°C.
Add 100 µl standard or sample to each well.
Incubate 2.5 h at RT.
Add 100 µl prepared streptavidin solution.
Incubate 45 min at RT.
Add 100 µl TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl Stop Solution to each well.
Read plate at 450 nm immediately.
Standard, Biotinylated Visfatin peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.