RayBio® Human/Mouse/Rat Pro-Neuropeptide Y/CPON EIA Kit optimized for serum and cell culture supernatants. Competition-based ELISA on a 96-well strip plate.
Uniprot Accession Number: P01303 Gene Symbol: NPY Gene ID: 4852 Protein Name/synonyms: Pro-neuropeptide Y [Cleaved into: C-flanking peptide of NPY (CPON)]
Target species: Human I Mouse I Rat Solid support: 96-well Microplate Solid support: Quantitative Result Output: Quantitative Method of Detection: Colorimetric Compatible Sample Types: Cell Culture Supernatants I Serum Sensitivity: 0.19 ng/ml or 16.59 pM Detection Range: 0.1-1,000 ng/ml Specificity: This kit was designed to recognize the C-terminus of pro-NPY and therefore will detect full-length pro-NPY and CPON. However, it does not recognize active forms including NPY 1-36, 2-36, 3-36 or 3-35.
This kit detects both CPON and the 69 aa pro-form of NPY.
Cross Reactivity: This EIA kit shows no cross-reactivity with any of the cytokines tested: Ghrelin, Nesfatin, Angiotensin II and APC.
Strip plates and additional reagents allow for use in multiple experiments
Quantitative protein detection
Establishes normal range
The best products for confirmation of antibody array data
Pre-Coated 96-well Strip Microplate
TMB One-Step Substrate
Positive Control Sample
Prepare all reagents, samples and standards as instructed.
Add 100 µl detection antibody to each well.
Incubate 1.5 h at RT or O/N at 4°C.
Add 100 µl standard or sample to each well.
Incubate 2.5 h at RT.
Add 100 µl prepared streptavidin solution.
Incubate 45 min at RT.
Add 100 µl TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl Stop Solution to each well.
Read plate at 450 nm immediately.
Standard, Biotinylated Neuropeptide Y peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.