VectaFluor™ Ready-to-Use (R.T.U.) DyLight® dye-labeled Secondary Antibodies offer maximum convenience for fluorescence staining of cells and tissues. Our affinity purified, extensively cross-adsorbed secondary antibodies are conjugated to DyLight® dyes in a manner that ensures maximum degree of labeling without compromising antibody affinity or specificity. DyLight® dyes offer several advantages such as brighter fluorescence, greater photostability, and pH independence.
VectaFluor™ R.T.U. Antibody Reagents are supplied as 15 ml pre-diluted, stabilized, ready-to-use solutions along with 15 ml ready-to-use 2.5% normal horse serum for blocking.
Excitation maximum is at 493 nm
Emission maximum is at 518 nm
DyLight® fluorescent dyes are direct alternatives to traditional fluorophores such as fluorescein (FITC) and rhodamine. The excitation and emission spectra parallel that of other commercially available fluorescent reagents allowing for easy substitution into an existing protocol without requiring any further instrumentation or filter sets.
DyLight® dyes offer a number of potential advantages including greater photostability and brighter fluorescence. DyLight® dyes are completely stable over a pH range of 4-9 making them compatible with many aqueous-based buffers and diluents. The DyLight® dyes can be applied as single labels or in combination with other DyLight® dyes and fluorophores as part of a multiple immunofluorescent antigen staining methodology. The Dylight® dyes currently offered are DyLight® 488 (green), DyLight® 549 (orange), DyLight® 594 (red), and DyLight® 649 (far red).